Phasing tiny crystals

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Phasing tiny crystals

For tiny crystals, their diffraction intensities at and between the Bragg peaks become measurable due to the limited number of the unit cells, which can in principle be used to directly phase the crystal structures.

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Merohedral or pseudomerohedral twinning of crystals cannot be identified from inspection of the diffraction patterns. Several methods for the identification of twinning and the estimation of the twin fraction are suitable for macromolecular crystals and all are based on the statistical properties of the measured diffraction intensities. If the crystal twin fraction is estimated and is not too c...

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MIR phasing using merohedrally twinned crystals.

Merohedral twinning is a crystal-growth disorder that seriously hinders the determination of macromolecular crystal structures by isomorphous replacement. The strategies used in the structures solved so far are discussed. Several methods can be used to determine the extent of twinning, the twin fraction and to detwin the data. Accurate determination of the twin fraction by analysing heavy-atom ...

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ژورنال

عنوان ژورنال: IUCrJ

سال: 2013

ISSN: 2052-2525

DOI: 10.1107/s2052252513034507